The trail can be accessed from multiple points along the trail, with the two primary access points being at either end of the trail in Biddeford and Kennebunk.
Biddeford access is from the parking lot of Southern Maine Medical Center (SMMC). Follow ME Route 111, east of the I-95 Exit 32 for 0.2 miles and turn right into SMMC. West Cole road is 0.4 miles from the Biddeford Exit at the second light, also on the right. Park in the back lot.
Kennebunk access is via the Kennebunk Elementary School located off ME Route 35 approximately 0.6 miles north of the I-95 Exit 25 in Kennebunk.
The off-road section connecting Biddeford, Arundel, and Kennebunk provides over six miles of trail for walking, running, biking, cross-country skiing, and snowshoeing. The trail passes through unique forests along the former Eastern Railroad bed. The trail is extremely flat, the surface is hard packed stone dust and is suitable for road bikes or mountain bikes. The trail crosses the Maine Turnpike in Kennebunk on a uniquely designed bridge that was opened in 2011.
The Eastern Trail is a 62 mile multi-use, non-motorized greenway, that connects Bug Light in Casco Bay, South Portland to Kittery, Maine. Approximately thirty percent of the trail is off-road, and the trail is a part of the East Coast Greenway which will eventually connect Calais, Maine to Key West, Florida, approximately 3000 miles. The Eastern Trail is currently composed of 21 miles of off-road trails on the former Eastern Railroad bed with the remainder 41 miles on public roads.